Not familiar with the "Jojo" series personally, but this is probably the most "badass," and one of the coolest depictions of the Cutie-mark Crusaders from MLP:FiM I've ever seen. Very amazing realistic detail on this one, especially the clothing wrinkles, and such. The muscular details are nice to, while also not making them look to muscular, and still a bit feminine. My only criqitue is the pony-like legs On Sweetie & Scoots seem to bend to far back, but it's still kinda neat you made their legs a bit like pony legs. Scootaloo's wings also seem a bit low, (I'm used to seeing people draw them closer to her shoulders) but you still did a great job on the feathers.
This might be your best anthro' and/or pony piece so far. Very good work.